Insertion junction: CLIP2.075266_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.075266
Chromosome:chromosome 3
Location:6043239
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g190000 PPP14 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTACACTGAGAGACCGCGCGGGTAACTTGAATTCCTTCCACTGCGCTT
Internal bar code:CCCAGCACGGTGGTCCCTGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1768
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCGCTACAGGATCTGAG
Suggested primer 2:TATCCAGAGAACCCCGCTGA