| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075272 |
| Chromosome: | chromosome 14 |
| Location: | 3859116 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632600 | (1 of 1) PF07572 - Bucentaur or craniofacial development (BCNT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGTGTCTGCCCCGTGAGTAACCGGGAGCGCCTTGCCCACATTACGTT |
| Internal bar code: | TAATTGTACTTTAACAGATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2413 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCACTGCCTCGAACCAG |
| Suggested primer 2: | ACAGGGACAGGGATAGGGAC |