| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.075278 |
| Chromosome: | chromosome 14 |
| Location: | 1032582 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614100 | GTR26,DGL1 | Dolichyl-diphosphooligosaccharide-protein glycosyltransferase; (1 of 1) K12670 - oligosaccharyltransferase complex subunit beta (WBP1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGGAAGGGAGGCAGCCTGTGGAGGCACCCTGTCCACCCTGCTACATC |
| Internal bar code: | GAGGGTGGGAATTTCCGACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1221 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAAGCATAAGCACGCTC |
| Suggested primer 2: | CCCACAGTCTACAGCCCTTG |