Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.075288 |
Chromosome: | chromosome 3 |
Location: | 5779034 |
Confidence (%): | 40 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g187600 | (1 of 15) PF13365 - Trypsin-like peptidase domain (Trypsin_2) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTACGTCTTTGCCTTCTTCGCTCTTAAGCCAGGGTGGGTTGCTTGAC |
Internal bar code: | GCTCTACGTATCTTTGATGGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 736 |
LEAP-Seq percent confirming: | 94.4444 |
LEAP-Seq n confirming: | 17 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCACGTCACCAGTAGACAA |
Suggested primer 2: | GGGTAAAGCAGCGCAACAAA |