| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.075289 |
| Chromosome: | chromosome 3 |
| Location: | 6431098 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193000 | HAD5 | Halo-acid dehalogenase-like hydrolase; (1 of 1) 3.1.3.29 - N-acylneuraminate-9-phosphatase | intron |
| Cre03.g193050 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTGTCGCTTCCCCTCACGGGCGCTGGAGCCTCTGCTTCAGAAAACAA |
| Internal bar code: | CGGAGGTTTGGGTATAGGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCATGATCACAGCAGC |
| Suggested primer 2: | GATGCTGCACACAAGTTGCA |