| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075290 |
| Chromosome: | chromosome 10 |
| Location: | 5225669 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456100 | FAP142,POC21,AGG3 | (1 of 4) K03809 - NAD(P)H dehydrogenase (quinone) (wrbA); Aggregation 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCCGTCATTTAGCGGCGGTTACAGACATGACGGCAACGGCAACGCCG |
| Internal bar code: | GTGCATTTGAAACTAAAGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4827 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGCGCGTTCTAATTAAGC |
| Suggested primer 2: | CCATGGCAAAGCGTGATAGC |