Insertion junction: CLIP2.075294_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.075294
Chromosome:chromosome 6
Location:2962783
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre06.g273650 (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGCGGGCGCGGCCTGAGCACTACGCCCCCCTCCCCGCCCCACCCGCG
Internal bar code:AAGGTGGAGCCAGGATGGTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:301
LEAP-Seq percent confirming:25.3165
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCTTGGAGCTGGGACTG
Suggested primer 2:TCGTTTAGCGCTCACACACT