Insertion junction: CLIP2.075316_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.075316
Chromosome:chromosome 2
Location:2851534
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094700 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCGCCGGTTGAACAGTCGACGGCGGCAACCATGAGCGGCACTGCCCG
Internal bar code:CGGCTGGGGGGACGACGCGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3241
LEAP-Seq percent confirming:82.6087
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CGACGCCGCAAACATTCTAG
Suggested primer 2:GGTTCCAACCTCTGCTTCGA