| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075324 |
| Chromosome: | chromosome 6 |
| Location: | 4756146 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279550 | (1 of 62) PF00226 - DnaJ domain (DnaJ) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTGCATGGGCTCGCTCATGTCCTTCCACACCAACTCCTTGGTCCTGC |
| Internal bar code: | GAAGAGCCTCTCGTTTCATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 123 |
| LEAP-Seq percent confirming: | 23.6842 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATCAAAAGGATCCAGCG |
| Suggested primer 2: | TTGCACATTGCCTTTGGACG |