Insertion junction: CLIP2.075336_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.075336
Chromosome:chromosome 13
Location:4486678
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g602901 CNX2 (1 of 1) PTHR22960:SF0 - MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1; Cofactor of nitrate reductase and xanthine dehydrogenase 2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTTGCTGAGATGCAGCCGGGCGCTACCATGCCTCCGCTGAACTTGTCA
Internal bar code:GTGACAGTTTTCGCTGTGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:143
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTGTGTTGCCCTTTTGC
Suggested primer 2:ACAATTGGCGGGTAGAGTGG