Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.075342 |
Chromosome: | chromosome 8 |
Location: | 3449191 |
Confidence (%): | 40 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g378450 | (1 of 1) IPR000104//IPR000679 - Antifreeze protein, type I // Zinc finger, GATA-type | 5'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGTGAGCAAGTTCCAGCTGCGTTGTGGCCCCCGTGGCACGCGTCGGGC |
Internal bar code: | GAGGTTGGATGTATAATGGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 146 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 1 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCTCAGGCATGGCTTGGAA |
Suggested primer 2: | AGAAGATGTTGTGGGCGAGG |