| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075349 |
| Chromosome: | chromosome 2 |
| Location: | 314104 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075050 | PTA1 | (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter | 5'UTR |
| Cre02.g075100 | TPR3 | (1 of 1) PTHR22904//PTHR22904:SF356 - TPR REPEAT CONTAINING PROTEIN // TETRATRICOPEPTIDE REPEAT PROTEIN 1; Tetratricopeptide-repeat protein 3 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTTTGCTGAGTCCAAACGCTGGCCCAACTTGGTGGCGGCGCCGGTAG |
| Internal bar code: | GAAATGCGAACCGACGGGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4458 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGGATTGGGGTTTGGG |
| Suggested primer 2: | TTACGTCATGTATCGGCGGG |