| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075351 |
| Chromosome: | chromosome 6 |
| Location: | 2247849 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266900 | LPA1L | Low PSII Accumulation 1 homolog; (1 of 2) PF11998 - Protein of unknown function (DUF3493) (DUF3493) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGAAGCTCGCACGCCTGGCAACCCTTGTTGTGATTCTTAAGCACGA |
| Internal bar code: | ATAAGATTTTGTAATCCCTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4748 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGTGTGAGGTGTCGACG |
| Suggested primer 2: | AACAGCTCACCGGAATGGAG |