Insertion junction: CLIP2.075357_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.075357
Chromosome:chromosome 4
Location:2967114
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g800478 (1 of 4) PF00385//PF00665 - Chromo (CHRromatin Organisation MOdifier) domain (Chromo) // Integrase core domain (rve) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGGCAGTCGCAGACTGGCCTACGCCGGCCAATGTGTCTGACTTGCGCT
Internal bar code:TGGGGTTCCCGGACGGTATAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2460
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAATCCGACTGATCCCGG
Suggested primer 2:AGGTTTCGATAGCTCGCTCG