Insertion junction: CLIP2.075357_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.075357
Chromosome:chromosome 4
Location:2967122
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g800478 (1 of 4) PF00385//PF00665 - Chromo (CHRromatin Organisation MOdifier) domain (Chromo) // Integrase core domain (rve) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGATCAGGGTGCACACCCTCGGCGGAAACCACGTGCCCCAAAAACGGCA
Internal bar code:ACTTTATTTTCCGAGCAAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2745
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTTCGATAGCTCGCTCG
Suggested primer 2:CTCAATCCGACTGATCCCGG