| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.075360 |
| Chromosome: | chromosome 17 |
| Location: | 3350934 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722900 | (1 of 781) IPR000104 - Antifreeze protein, type I | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCGCAGGTGTTGCGGGAACAGTTGCGGGAGACACCTGCTGACCCGC |
| Internal bar code: | GTGCGGGTGACTGTTATAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 4.7619 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGTTCCTCGCGCTAGTTT |
| Suggested primer 2: | TCACAGCGCCACTCGTAATT |