| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.075363 |
| Chromosome: | chromosome 16 |
| Location: | 7393985 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680454 | (1 of 8) PF13417 - Glutathione S-transferase, N-terminal domain (GST_N_3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGGGAGCCGTTCCCGAGCTTGTCTCGCTCAGCATCTCAGCGTGGTCC |
| Internal bar code: | GGCTACATTAGGATGTAACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1804 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAAAACGGGCACTGCAT |
| Suggested primer 2: | CACCTAACCCGCCCAAATCT |