| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075368 |
| Chromosome: | chromosome 12 |
| Location: | 2960513 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502200 | (1 of 1) K03240 - translation initiation factor eIF-2B subunit epsilon (EIF2B5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAAGGCACGCAGGAGCGTGTATGCGATGGTGCGTGGGGAGGTAGGCG |
| Internal bar code: | CGCGTTCGATGTGCGGAGTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAGGGTGAGGCTAGAGG |
| Suggested primer 2: | CTACGCCTGCTGTCTACCTG |