| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075369 |
| Chromosome: | chromosome 12 |
| Location: | 4065281 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516550 | GTR16,BMT1 | (1 of 1) 2.4.1.142 - Chitobiosyldiphosphodolichol beta-mannosyltransferase / mannosyltransferase; putative Beta-1%252C4-mannosyltransferase | 3'UTR |
| Cre12.g516600 | STK25 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Serine/threonine protein kinase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGGAAGGGACGACAAAGTGCAAGCCTGCAATGACCGTGCCGTGGCTG |
| Internal bar code: | CATAAATTGGGTTTCGAGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4861 |
| LEAP-Seq percent confirming: | 67.2269 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 119 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGTACGCTGCGATTCTG |
| Suggested primer 2: | GCCGCAGAAGGAGATGTACA |