Insertion junction: CLIP2.075373_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.075373
Chromosome:chromosome 13
Location:5207605
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g607600 FSN1,RIBFL1 Similar to riboflavin biosynthesis protein RibF; (1 of 1) PTHR22749//PTHR22749:SF0 - RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE // RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBF 3'UTR
Cre13.g607650 POC15 Proteome of centriole protein 15; (1 of 1) PTHR10891//PTHR10891:SF5 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGAACTCCTTCACCAACAAGCCTTGCACAGTGCTCATCTCTACCACGA
Internal bar code:TGGTATAGTCTGGCCTTGCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:5441
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:92

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGAGACGCTGAGCCTCTA
Suggested primer 2:AGACTTCGATGCATGCAGCT