Insertion junction: CLIP2.075395_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.075395
Chromosome:chromosome 3
Location:9083350
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g201776 (1 of 1) IPR006575//IPR016135 - RWD domain // Ubiquitin-conjugating enzyme/RWD-like 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAAATGTCATGGGCGGAACTCGTAAGAGTGCGGAAAGCGTGATCCATCG
Internal bar code:AATAGCGGGCACTCAATTGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:393
LEAP-Seq percent confirming:7.14286
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGTGACTTTGTGAGGCGC
Suggested primer 2:AAGGCTCCCAAGTTCACCTG