| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.075397 |
| Chromosome: | chromosome 10 |
| Location: | 5204646 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455850 | MCM7 | (1 of 1) K02210 - DNA replication licensing factor MCM7 (MCM7, CDC47); Minichromosome maintenance protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACACTCACACAGTTGAAGTAAGCGGCATTTCGGGAAACCGCGG |
| Internal bar code: | GGTTCTATGCCAGTGCAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGAGAGTGAGATGGGGT |
| Suggested primer 2: | GACGTCCTCCATGCCAAAGA |