Insertion junction: CLIP2.075398_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.075398
Chromosome:chromosome 6
Location:8514704
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g800754 (1 of 5) K11446 - histone demethylase JARID1 [EC:1.14.11.-] (JARID1) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGTTGTCCACCTTCAGCTTCTTGATGTAAACGTAGTCGCCAGCGCTGA
Internal bar code:AGTATTGTAGCAGCGTCCGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3907
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TCATCTGTTGGGGCGTCTTC
Suggested primer 2:GCACGCATGGTCAATACGTC