Insertion junction: CLIP2.075407_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.075407
Chromosome:chromosome 1
Location:8075312
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g063267 BLP1 Bystin-like protein; (1 of 1) K14797 - essential nuclear protein 1 (ENP1, BYSL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTTGACCAGGCAGACACTGAAGGGATCCATAGCGCAGAATTGACAGGA
Internal bar code:TGTGTTAGGATGCACACTGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2000
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTGCCCATCGCTATTGGC
Suggested primer 2:ATGTATGCATGTTGACGCGC