Insertion junction: CLIP2.075412_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.075412
Chromosome:chromosome 1
Location:4245719
Confidence (%):40
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTTCGCTTCTGGGTGCGCTGGTAGGTCAACGACAAGTACCACAAGTTC
Internal bar code:AGACGCCCAAAGTCCGAATTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:894
LEAP-Seq percent confirming:6.45161
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCCGCTTCCGAAACATC
Suggested primer 2:GCGTGAGATAGTACAGGGCC