Insertion junction: CLIP2.075415_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.075415
Chromosome:chromosome 5
Location:3078110
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre24.g755547 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCTCATCAACACTCCCACGCGACGGTGGTGGGCGCCTAGTCCTCCTC
Internal bar code:GCCATGACAATAAATTTTTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:681
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGGGTTGGTGATAGGTGC
Suggested primer 2:TTCGACGTGGGAGGGAAAAG