| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075415 |
| Chromosome: | chromosome 5 |
| Location: | 3078143 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755547 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGCGCCTGCGGGTGCTGGGGGCGCCTTGTAAGATTCGAGCAGGGACT |
| Internal bar code: | GCCATGACAATAAATTTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2655 |
| LEAP-Seq percent confirming: | 92.3404 |
| LEAP-Seq n confirming: | 217 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 235 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGACGTGGGAGGGAAAAG |
| Suggested primer 2: | TGTGGGTTGGTGATAGGTGC |