Insertion junction: CLIP2.075432_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.075432
Chromosome:chromosome 13
Location:2545919
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g580250 (1 of 1) PF03941 - Inner centromere protein, ARK binding region (INCENP_ARK-bind) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAACAACCGTACGATCTCAGCCTCCCCCGGCCTCAACAAACAAGGGCC
Internal bar code:GCTCATTGGTTTGTCTTAATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:272
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCACCTGGTCACATGACTGC
Suggested primer 2:TGGTGGAGTGCTGTGTTTGT