| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075433 |
| Chromosome: | mitogenome |
| Location: | 12171 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreMt.g802344 | ChrepMp08,801489,rtl | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1); reverse transcriptase-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATATTAAGCACTTGATGCAGACTCTACAGTTGCAATACCTTCCCAATGA |
| Internal bar code: | GTGCGAGACACGGCGTGATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2189 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCCATCGATAGGAACTC |
| Suggested primer 2: | GCTAGGGGTGAAAGGCCAAT |