Insertion junction: CLIP2.075437_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.075437
Chromosome:chromosome 9
Location:2906345
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g390500 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAGCATGTGGAGCAGCTGGGGGCCCTGCGCTGGCTGCTGACACACGCG
Internal bar code:CGCGTAATGGTTTGAAATACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:110
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACCTGCAAATCAAACCA
Suggested primer 2:AGCAAAATGTTCCGCAACCC