| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.075443 |
| Chromosome: | chromosome 4 |
| Location: | 2444800 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221650 | TOP4 | (1 of 1) PTHR10169//PTHR10169:SF48 - DNA TOPOISOMERASE/GYRASE // DNA GYRASE SUBUNIT A, CHLOROPLASTIC/MITOCHONDRIAL-RELATED; Topoisomerase type IV | 5'UTR |
| Cre04.g221700 | COX3 | Mitochondrial cytochrome c oxidase subunit III; (1 of 1) K02262 - cytochrome c oxidase subunit 3 (COX3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGAATTAGTTTAGGGGCTTTTCGTCAAGGTCTTTGCAAGTCTTTTGC |
| Internal bar code: | GGGGTTGATCGTGGCAAAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2468 |
| LEAP-Seq percent confirming: | 98.4375 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCTTACCTTGCCGAAGC |
| Suggested primer 2: | GTTGTTGGCAGTGAAGCTGG |