| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075452 |
| Chromosome: | chromosome 2 |
| Location: | 6754732 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387726 | AST1 | Aspartate aminotransferase; (1 of 1) K14454 - aspartate aminotransferase, cytoplasmic (GOT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACACATACCACCACCAGGCAAGACCACCACGCCACAAAAAGGGTTT |
| Internal bar code: | GGGTATGCGTATTATAAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 63.3333 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAAACACTCCGCCCCTAC |
| Suggested primer 2: | TTGACAAGGCGGAAGAGGTC |