| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.075455 |
| Chromosome: | chromosome 6 |
| Location: | 3723280 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278160 | PRL5 | (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGACTGTGCTTCGACAAGTGGAACTGATCAGCTAAGCACACTGGCTTG |
| Internal bar code: | CGTGACGGGGTCCTGGTCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 897 |
| LEAP-Seq percent confirming: | 63.3333 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGGTATTCATAGGCGCG |
| Suggested primer 2: | GCTATACCCCTGTGCCGTAC |