Insertion junction: CLIP2.075460_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.075460
Chromosome:chromosome 6
Location:2952318
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre06.g273450 POB11 Proteome of basal body 11 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAAGATGTCCGAGGTGCGTGACTGCGCGCGCTCATGCACCGTGTTGTCC
Internal bar code:CATGTAGAGACACGTATCCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1696
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GCCACCCAACACATGTTTCC
Suggested primer 2:GCTTGAGGACGTCTATCCGG