| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075462 |
| Chromosome: | chromosome 13 |
| Location: | 2889995 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583300 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAACAATACTTACATGCATCCCAAACGTGTGTCATTTGCCAGCACCCA |
| Internal bar code: | GGTATTGGTGTACGTGGTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGAGAAAGCCCGCTTGAG |
| Suggested primer 2: | GTTGCTGTTGGACTTCGCTG |