| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075475 |
| Chromosome: | chromosome 7 |
| Location: | 1339883 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322550 | FAP240 | Flagellar Associated Protein 240; (1 of 1) PF14977 - FAM194 protein (FAM194) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATATCGCGTATACCGGTAGTGGACCTTTCCACCCCATTCACATTGTGG |
| Internal bar code: | ACTACGTGCATTTGACCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3476 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGCTTGCTGACTGTGCT |
| Suggested primer 2: | CCCCTTTTATGTCCGCACCT |