| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.075487 |
| Chromosome: | chromosome 2 |
| Location: | 660358 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077800 | CSE13,FAP310 | Flagellar Associated Protein 310 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCGCCCATGCGCCCTCCGTCTGACCTGGTCTGACCGTCCGCCCGCCG |
| Internal bar code: | AGGGGATCTCTGTTCAAGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2133 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTCTCTCAAGTTGGCAG |
| Suggested primer 2: | TGCTAGGACCGTTGTTCCAC |