| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075495 |
| Chromosome: | chromosome 9 |
| Location: | 3811305 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397734 | (1 of 2) K07424 - cytochrome P450, family 3, subfamily A (CYP3A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCATGCGGCGGCTGCCCGGGCGGCCAGCACCACTGCCCAGACTCCCCA |
| Internal bar code: | CATGAAGCGCTATGGCTAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 35.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTACGGCATGATTGTGCG |
| Suggested primer 2: | CTCTTGTACGGGCTGGACTG |