| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075511 |
| Chromosome: | chromosome 6 |
| Location: | 7300031 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAAAGATTTACGTAGGTCTAGATAGGCGGCTGCAGGACAGCAGGGAT |
| Internal bar code: | GATGAGATTGACGTAACGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2821 |
| LEAP-Seq percent confirming: | 94.6429 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTACCTGCACCTTCCAC |
| Suggested primer 2: | CAGGGCGTCTGAATTGCATG |