| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075531 |
| Chromosome: | chromosome 16 |
| Location: | 1303427 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651450 | (1 of 3) PTHR13367:SF8 - OTU DOMAIN-CONTAINING PROTEIN 7B | 3'UTR | |
| Cre16.g651500 | (1 of 1) K12180 - COP9 signalosome complex subunit 7 (COPS7, CSN7) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGGCTGATCGGGTACTGAGTCAATTGACAGGATTGCCCCCCCCCCTCC |
| Internal bar code: | CTGGCGCTAGATTCAGTACCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1223 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTGCCGTGAATGCTGTG |
| Suggested primer 2: | CAGGTGTGTGACGTTGCTTG |