| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075555 |
| Chromosome: | chromosome 6 |
| Location: | 7741075 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302850 | 5'UTR | ||
| Cre06.g302900 | (1 of 1) PTHR23180//PTHR23180:SF203 - CENTAURIN/ARF // CENTAURIN BETA 1A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCTTGCGGAGCGCGTGTAGTGTCCTACGCAAAATGTGCGATGCCCGT |
| Internal bar code: | AGTCTTATCTCTCGCGTCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2959 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGCACAGCGTTGAAAC |
| Suggested primer 2: | CCTTCACCTCAGCTTCAGGG |