| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075557 |
| Chromosome: | chromosome 10 |
| Location: | 5503379 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457950 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACCACTGTTTGATTTGCACCACTGTTTATAGGTACTGCCACAAACA |
| Internal bar code: | GCCAATCATGGCTGGGATTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1537 |
| LEAP-Seq percent confirming: | 90.625 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGATCAAACGTCGCAATT |
| Suggested primer 2: | ATGTTCGAGCTGCACACTGA |