| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075560 |
| Chromosome: | chromosome 12 |
| Location: | 4468817 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520350 | ALDH12A1,ALD1,ALDH12 | (1 of 1) 1.2.1.88 - L-glutamate gamma-semialdehyde dehydrogenase / Pyrroline-5-carboxylate dehydrogenase; Aldehyde dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGATTTGCGGGATGCTCCACCTCAAAGCAGCCCAGAACTGGGACCCCT |
| Internal bar code: | TAGCATGTCTGATTTAGTACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1843 |
| LEAP-Seq percent confirming: | 97.3684 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGCATCCTTCTTGCTGG |
| Suggested primer 2: | ATGACCAAAACGCAACGTCG |