| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.075562 |
| Chromosome: | chromosome 15 |
| Location: | 577928 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636450 | SMM58 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) K15190 - 7SK snRNA methylphosphate capping enzyme [EC:2.1.1.-] (MEPCE, BCDIN3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTTTCGGCCGTCATGCGAGCTGCGTCTTGACTTAGCTTACTTGCGCT |
| Internal bar code: | TTCAACGGGTTATTGGACATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAATTGGAACGCTGTGTG |
| Suggested primer 2: | ATCCATCTCTTGGCAGGCAC |