Insertion junction: CLIP2.075566_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.075566
Chromosome:chromosome 13
Location:5036416
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g606350 (1 of 1) PF12816 - Golgi CORVET complex core vacuolar protein 8 (Vps8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGGGCTGTGGTGGTTAGTGGTCAGGGTGACTTGAAGAACGCACCTTGA
Internal bar code:TATGGTACTCCACGTCCTAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:209
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACGCTTCCAACCAACAC
Suggested primer 2:TGTCCGCGTCTGAGTTTTGA