| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075567 |
| Chromosome: | chromosome 6 |
| Location: | 3141248 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275650 | RPN3 | (1 of 1) K03033 - 26S proteasome regulatory subunit N3 (PSMD3, RPN3); 26S proteasome regulatory subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCCATTCCTTGAACCAAGAAAATGCACGCGGCTTGCCGTTCGCACG |
| Internal bar code: | GTGGAACATCGGTGTGCCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACGAGGACGACTTCTGA |
| Suggested primer 2: | AACTCGGTCAGCTCCACTTG |