Insertion junction: CLIP2.075569_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.075569
Chromosome:chromosome 12
Location:4055955
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g516450 CAG1 Gamma carbonic anhydrase 1; (1 of 1) PTHR13061//PTHR13061:SF7 - DYNACTIN SUBUNIT P25 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAGCTTATATTGCCAACAACGATAGCTTTGGAATTACAATGGCGTGA
Internal bar code:ACTTGGGTTCCACTAAATTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1715
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCACGTCCACTTACAGGT
Suggested primer 2:AGGGCATCATGAAGGTGCTC