| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.075582 |
| Chromosome: | chromosome 9 |
| Location: | 5733394 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410600 | PPP34 | (1 of 3) K17499 - protein phosphatase 1G [EC:3.1.3.16] (PPM1G, PP2CG); Phosphoprotein phosphatase 2C-related | CDS |
| Cre09.g410650 | HIS1 | (1 of 1) 2.4.2.17 - ATP phosphoribosyltransferase / Phosphoribosyl-ATP pyrophosphorylase; N-5-phosphoribosyl-ATP transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCAGAAGTATATGGCCGCGGAGCTGCAGCGGTTTGAGGAGTTTGGCA |
| Internal bar code: | TCCCCATGCAAGTGTGAGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAAAAGCTCGTTGCCGA |
| Suggested primer 2: | CACGGACTACAAAGGACCCC |