Insertion junction: CLIP2.075590_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.075590
Chromosome:chromosome 13
Location:1570855
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g573050 (1 of 1) K14769 - U3 small nucleolar RNA-associated protein 11 (UTP11) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCGGTATATGCAAGGCAAAGCCAAGACTTAGGGTTATCATGGACGCT
Internal bar code:TAGTTTCGCCCTAACCATTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1647
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCGAAATAGGGACGAAAC
Suggested primer 2:GCGCTTTCTCTGCCACAAAA