| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075591 |
| Chromosome: | chromosome 12 |
| Location: | 2730412 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504550 | HTB15 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGGGCTCCTTGGCAGCCTTCTTGGAGGGCTCCTTGGCCTTCTTGGCG |
| Internal bar code: | GACCCACCCCGAACTGTCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2630 |
| LEAP-Seq percent confirming: | 33.0097 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCCTTCTTCAGGTAGCG |
| Suggested primer 2: | GCTGCTTTCCACGCTTTCAA |